Protein Purification

In this case study we present our ability to express, phosphorylate and separate the native (dephosphorylated form) and the active (dual phosphorylated form) of a kinase to produce batches at high yield with excellent purity. Many serine/threonine kinases are involved in  the regulation of [...]

Our latest achievement: the successful expression, purification, and crystallization of Cereblon-midi (CRBN-midi) reinforces Sygnature Discovery’s position at the forefront of the rapidly expanding targeted protein degradation (TPD) landscape. Transforming Drug Discovery Targeted protein degradation is redefining how scientists approach diseases once considered “undruggable.” Instead [...]

The baculoviral expression vector system (BEVS) was first introduced in the 1980s. It is widely used to generate many types of protein including intracellular, secreted and membrane proteins particularly GPCRs. As BEVS is a eukaryotic system it is capable of many post translational modifications, [...]

In this case study we describe the journey to develop a process for the production of the protein component of a biopharmaceutical that was suitable for full scale manufacture. The initial laboratory scale process we were presented with was not fit for purpose and [...]

In this case study Toby Allen demonstrates the value of using multiple analytical methods to characterise Intrinsically Disordered Proteins (IDPs) and highlights some of the techniques we have available at Sygnature Discovery. What are IDPs: While many globular proteins have been characterised with a [...]

In this case study, Rachel Johnson describes how we determined the active state structure of the 5-HT2A receptor with the natural ligand serotonin bound using cryo-EM. Introduction GPCRs are a large and diverse group of receptors in the body. They are also important therapeutic targets [...]

One of our Senior Protein Scientists, Sam Bloor, describes the journey to develop a refolding route for an interleukin. Introduction Cytokines are important cell signalling proteins which bind to cell surface receptors, initiating downstream cascades 1. They have a range of functions such as [...]