Protein Science

A client recently requested the production of two bispecific antibodies and provided us with the heavy and light chain variable sequences that they required. After reviewing the literature, we decided to produce these bispecific antibodies as fused, single-chain variable fragments (scFvs) with an N-terminal [...]

Biotinylation of proteins is a powerful tool for protein science and our recent case study on the in vivo biotinylation of intracellular proteins by co-expression with BirA ligase described how this method is now used routinely at Peak Proteins. A number of our mammalian expressed [...]

Our client needed a biotinylated version of a highly disordered protein for their high-throughput screening assay. Previous work with the protein of interest had demonstrated issues with protein stability and loss of protein during concentration using centrifugal concentrators; we were therefore concerned about further [...]

We had been asked by a client to express and purify a protein:Fab complex to support a Cryo-EM project. This was just to be a reproduction of a crystal structure that had been reported in the literature and so on the face of it [...]

Many proteins exist and function as multimers in their native state. However, the mechanism by which they achieve this can prove to be an issue when trying to express and purify the protein recombinantly. Two recent projects we have been involved with, required solutions to [...]

Early on in any recombinant protein expression and purification project, there are many factors that we at Peak Proteins consider as part of the protein construct design process. It cannot be understated the importance of committing time and effort to this stage, as the [...]

A client requested the large-scale production of a protein that had previously been optimised for purification during feasibility studies. This protein had proven tricky to purify during the feasibility stage and two attempts had been taken to establish a suitable purification strategy. High protein [...]

A client requested assistance providing protein to enable a high throughput screen (HTS). This was for an emerging target that was implicated in the development of neurodegenerative diseases, strokes, and epileptic seizures.  Their yields of purified protein from E.coli were around 1mg/L, with initial [...]

A client requested the production of biotinylated and non-biotinylated heterotrimeric complex to support assay screening and biophysics. The protein complex plays a role in tumour suppression, and thus can be used to identify novel targeted therapies. Proteins A and B were cloned into [...]

At Peak Proteins we regularly design DNA constructs for clients, for insertion into mammalian, insect, and bacterial cell systems for over-expression of intracellular proteins. The expressed proteins then need to be isolated and purified to be supplied for a variety of uses determined [...]