We can express your protein in E. coli, baculovirus/insect cells and mammalian cells.
Using our wealth of experience, we manipulate the standard T7 based expression vector systems in E.coli to maximise soluble expression. Where necessary we are able to refold from E.coli derived inclusion bodies. Our strong publication record in this area demonstrates this with both cytokines and proteases.
Of course, E.coli is not suitable for many proteins. We also use the baculovirus/insect cell system, working with the flashBACTM technology from Oxford Expression Technologies. This uses recombinant virus generated very simply with a single co-transfection of two plasmids directly into insect cells. Again, this builds on substantial experience working with different insect cell expression systems.