All of the analysis is performed on a Sciex ExionLC coupled to a Sciex X500B QTOF mass spectrometer with Sciex OS and BioPharmaView Software. Details of each service we provide are:
Intact protein molecular weight analysis (LCMS)
- Accurate intact protein mass analysis up to 150KDa, requiring 1-10 mg of protein
- Identification of post translational modification mass additions
- Covalent compound analysis
- A full results report is produced including all deconvoluted mass data
Peptide mapping and protein identification (LCMSMS) – so good they named it twice!
- Identification of single proteins or moderate complex mixtures
- Sequence and PTM confirmation of bespoke recombinant proteins
- Use of multiple proteases to maximise sequence coverage
A full results report is produced including all Mascot database matches for peptide mapping along with unique sequence confirmation using BioPharmaView software.