We now provide intact protein and peptide mapping as a stand-alone service to anyone requiring detailed protein or antibody characterisation.  Key to this service is the flexibility and quick turnaround times we offer with no detailed contract set-up.

Understanding the quality and quantity of any protein sample is key to obtaining decision making data. As part of our protein analysis package for protein reagents, we routinely perform LCMS to fully understand the heterogeneity, molecular weight and any post-translational modifications (PTM) that may have occurred during expression.

All of the analysis is performed on a Sciex ExionLC coupled to a Sciex X500B QTOF mass spectrometer with Sciex OS and BioPharmaView Software.  Details of each service we provide are:

Intact protein molecular weight analysis (LCMS)

  • Accurate intact protein mass analysis up to 150KDa, requiring 1-10 mg of protein
  • Identification of post translational modification mass additions
  • Covalent compound analysis
  • A full results report is produced including all deconvoluted mass data

Peptide mapping and protein identification (LCMSMS) – so good they named it twice!

  • Identification of single proteins or moderate complex mixtures
  • Sequence and PTM confirmation of bespoke recombinant proteins
  • Use of multiple proteases to maximise sequence coverage

A full results report is produced including all Mascot database matches for peptide mapping along with unique sequence confirmation using BioPharmaView software.

OTHER SERVICES

Protein Expression and Purification

We design, express and purify customised proteins reagents to support all stages of the drug discovery process. Our cell and protein scientists have a wealth of experience, many from large pharma organisations. We have many peer reviewed publications describing these techniques. We apply all our expertise and knowledge to your protein, providing an interactive, innovative and flexible approach to every project. Every protein and end use is different. We provide a tailored service, taking the time to understand your specific needs to deliver success. We express proteins using a range of platforms including mammalian HEK293 suspension cells, baculovirus/insect cells, and E. coli.

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Protein Crystallography and Structure Determination

Our protein structure determination service is driven by an outstanding team of experienced crystallographers with a strong publication record in solving a wide variety of protein structures, including novel ones. A key part of this process is the design of the protein and the ability to screen many different constructs and crystallisation conditions. Our final delivery package is not just a set of co-ordinates. We supply a detailed analysis of the overall 3D structure, along with our learning and conclusions, with ideas for project progression and drug design. Interactive discussions and regular communication with our clients are a key part of our service.

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GET IN TOUCH WITH PEAK PROTEINS

For further information or simply to say hello please contact us on 01625 238892 or email us