We were able to express our own nuclease enzyme, as a tool protein for in-house use. Protein expression as inclusion bodies in E.coli allowed us to generate large quantities of protein which we were then able to refold and purify to a high purity. Analysis of the final product indicated that the protein was correctly folded, and this was confirmed when tested for nuclease activity. We now have a broad specificity nuclease, which makes cell lysis and clarification easier, and with fewer problems for downstream processing and chromatography, improving yields. We are able to produce this cheaply, and use at scale.