We design, express and purify customised soluble and membrane proteins reagents to support all stages of the drug discovery process. We apply all of our expertise and knowledge to your protein to provide an innovative, interactive and flexible approach to every project.

Every protein and end use is different. We provide a tailored service, taking the time to understand your specific needs to deliver success.

  • Protein Design – is critical for success whether the end use be screening, structural biology, biophysics or in vivo. Guided by bioinformatics and modelling packages we explore different protein domains, point mutations and affinity tags and chimeras to produce the best solution.
  • Choice of mammalian, insect or E.coli expression systems – We routinely use HEK293 suspension cells for both secretory and intracellular protein expression as well as use the flashBACTM baculovirus/insect cell system licensed from Oxford Expression Technologies.  HEK293 cells in particular produce the most authentic post-translational modifications for human proteins which is key for any protein expression service. E. coli are frequently used for the speed and quantity of protein that can be achieved when a particular protein expresses well. We use T7 based expression vector systems to maximise soluble expression and can refold proteins from E. coli-derived inclusion bodies, if required.
  • Protein purification service – we use standard affinity chromatographic procedures with a variety of different tags. The design and selection of the most appropriate affinity tag can have a significant impact on the purification process and protein yields achieved. In addition, proteins are purified according to size, charge, hydrophobicity and other properties depending on the protein characteristics. A suite of Akta systems are available for the work.
  • Quality control package – As part of our quality control,  proteins are analysed by a variety of methods; SDS-PAGE under both reducing and non-reducing conditions as required, purity and size in solution, using size exclusion chromatography and protein concentration measuring absorbance at 280nm. Liquid chromatography mass spectrometry is used for intact mass of the protein and detection of any post-translational modifications and tryptic peptide mapping, if required. The thermal stability of the protein can also be monitored if needed.

GET IN TOUCH WITH PEAK PROTEINS

For further information or simply to say hello please contact us on 01625 238892 or email us