Protein Purification

Hear from one of our experts

Mark Elvin: Head of Cell Science

The Protein Science is the largest of our teams at Peak Proteins and between us we have tackled the purification and analysis of many diverse protein targets. The more challenging, the better….If there is a solution to a protein problem to be found, we will move mountains to find it.

Raquel Faba Rodríguez: Lead Protein Scientist

Summary of what we provide

If what you need is not listed below then please contact us, as it is likely that one of our team has experience with the technique you require.

PROTEIN EXPRESSION icon

Protein Construct Design

  • Guided by bioinformatics and modelling.
  • Affinity tags to aid expression, purification and to meet end use requirements.
  • Chimeras, mutations, truncations, deletions to meet project needs.
  • IMPROvER software for membrane protein stability.
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Protein Purification

  • Native or recombinant source.
  • Protein secreted, periplasmic, cytosolic or from inclusion bodies.
  • Cell lysis and clarification.
  • Column chromatography: all standard media and bespoke affinity columns.
  • Refolding from inclusion bodies.
  • Protein complexes.
  • 15N, 13C, 2H Labelled to support NMR.
  • Post translational modifications (e.g. biotinylation, phosphorylation).
  • Tag cleavage.
  • Low endotoxin batches with measurement using LAL assay.
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Membrane proteins

  • Wide range of classes: GPCRs, Solute transporters, ATPases, Ion pumps and channels.
  • Presentation: Detergents, SMALPs (detergent free co-polymers), Nanodiscs, Liposomes.
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Quality Control / Analysis

  • Sygnature Discovery Page
  • Absorbance wavescan.
  • Analytical size exclusion chromatography.
  • Intact and peptide mapping mass spectrometry.
  • Endotoxin levels via limulus amebocyte lysate (LAL) assay.
  • Bradford assay.
  • Thermal stability melting curves.
  • Flow induced dispersion analysis (Fida).
  • Protein NMR

Protein Purification Resources

TitleContentCategoriesResources Typeshf:categorieshf:tax:resource_type
Plunging into Cryo-EM: Precision Sample Prep for Structural Success, , , BlogMOREblog cryo-em protein-purification videosblog
Cautionary tales of secreted proteins in baculovirus infected insect cellsCase StudyMOREcase-study
Developing a robust biopharmaceutical production processCase StudyMOREcase-study
Characterising Intrinsically Disordered ProteinsCase StudyMOREcase-study
Determining the Cryo-EM Structure of the 5-HT2A receptor with Serotonin BoundCase StudyMOREcase-study
PIPS Placement Working on Targeted Protein Degradation, , Blog, Company NewsMOREfida blog protein-purificationblog company-news
Intrinsically Disordered Proteins as Drug Discovery Targets, , , MOREblog peak-favourites protein-expression protein-purification
Development of a Refolding Protocol for an InterleukinCase StudyMOREcase-study
Navigating the Peaks: A journey into protein science at Peak Proteins, BlogMOREblog protein-purificationblog
Biotinylation of recombinant proteins by co-expression with BirA in a range of different cell hosts, PostersMOREprotein-expression protein-purificationposters
Applications of FIDA in Protein Science, , PostersMOREfida membrane-proteins protein-purificationposters
Production of Active Cereblon and Analysis by SPR, PostersMOREprotein-expression protein-purificationposters
Revolutionising our Protein Science: How FIDA Enhances Protein Projects at Peak Proteins, , BlogMOREfida blog protein-purificationblog
Using the Power of Cryo-EM for Structure Determination at Peak Proteins, , , BlogMOREcryo-em membrane-proteins blog protein-purificationblog
Rapid Assessment of Membrane Protein Quality by Fluorescent Size Exclusion Chromatography, , , , Blog, Company NewsMOREpeak-protein-news membrane-proteins blog peak-favourites protein-purificationblog company-news
Options for Measuring the Concentration of Protein Samples, BlogMOREblog protein-purificationblog
Other Assays To Determine Protein ConcentrationColorimetric and fluorescent assays for protein concentrationProtocolMOREprotein-purificationprotocol
Determining Protein Concentration by Amino Acid AnalysisHow to calculate the concentration of a protein sample using AAAProtocolMOREprotein-purificationprotocol
Biotinylation of Recombinant Proteins, , BlogMOREblog protein-expression protein-purificationblog
Protein Scientist, Company NewsMOREpeak-protein-news protein-purificationcompany-news
Senior Protein Scientist, Company NewsMOREpeak-protein-news protein-purificationcompany-news
Alphafold: (Nearly) One Year On, BlogMOREblog protein-purificationblog
Peak Proteins Exhibits at PEGS 2022, EventsMOREevents protein-purificationevents
Inclusion bodies, enemy or ally?, BlogMOREblog protein-purificationblog
What’s ‘Hot’ to Like About Thermophilic Proteins?, BlogMOREblog protein-purificationblog
The Two Sides of Codon Optimisation, BlogMOREblog protein-purificationblog
The Origins of Recombinant Proteins, BlogMOREblog protein-purificationblog
Mutations, Mutations, MutationsThe mutations seen in the COVID-19 Variants of Concern The coronavirus disease-19 (COVID-19) is a respiratory disease responsible for the …, MOREblog protein-purification
Dynamic Assay for Profiling Anti-SARS-CoV-2 Antibodies and Their ACE2/Spike RBD Neutralization CapacityPeak Proteins are delighted that this paper, authors including our CEO, Dr Mark Abbott and Protein Purification Specialist Dr Raquel …, BlogMOREblog protein-purificationblog
Nature Webcast: How to Make Membrane Proteins and Not Unfold Them, , EventsMOREpeak-protein-news events protein-purificationevents
MolViewMolecule drawing and visualisation toolLinksMOREprotein-purificationlinks
MEROPS The Peptidase DatabaseThe MEROPS database is an information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit themLinksMOREprotein-purificationlinks
Protein Solubility Predictor ToolThe protein-sol software will take a single amino acid sequence and return the result of a set of solubility prediction …, LinksMOREmembrane-proteins protein-purificationlinks
Centrifuge Rotor Speed Calculator ToolVery comprehensive tool for calculating RCF values for most of the available centrifuge rotors, LinksMOREprotein-expression protein-purificationlinks
IMPROvER ToolIntegral Membrane PROtein stability sElectoR. Software that uses three different approaches to identify and rank potentially stabilising mutations in α-helical membrane proteins., LinksMOREmembrane-proteins protein-purificationlinks
MUSCLE Protein Sequence Alignment ToolMultiple protein sequence alignment toolLinksMOREprotein-purificationlinks
ExPASy Translate ToolTool to translate DNA sequences in all 6 frames, LinksMOREprotein-expression protein-purificationlinks
ExPASy ProtParam ToolTool for calculating the properties of your protein sequence (molecular weight, pI, extinction coefficient etc)LinksMOREprotein-purificationlinks
Biophysical Methods for Studying Human GPCR Ligand Binding Properties Poster Commentary, VideoMOREprotein-purification videosvideo
Measuring Coronavirus Spike:ACE2 Binding Affinity and Kinetics, VideoMOREprotein-purification videosvideo
UniProtUniProt provides the scientific community with a comprehensive resource of protein sequence and functional information.LinksMOREprotein-purificationlinks
Western Blotting ProtocolA tried and tested method for Western blots from PAGE.ProtocolMOREprotein-purificationprotocol
Protein Tag and Leader Sequence InformationSummary of commonly used tags for protein purification and Secretory leader sequences used for expression in either insect or mammalian …, , ProtocolMOREprotein-purification protein-construct-design protein-expressionprotocol
Protein Construct Naming ConventionOur in house convention which aims to standardise construct naming and thereby reduce confusion., ProtocolMOREprotein-purification protein-construct-designprotocol
Determining Protein Concentration by UVAn in depth look at UV absorbance and the equation required to measure protein concentration., ProtocolMOREprotein-purification protein-expressionprotocol
Protease InformationProteases are used as tools in protein biochemistry and mass spectrometry experiments. This protocol gives information about their use., ProtocolMOREprotein-purification mass-specprotocol
Fluorescent Protein TagsSummary information about fluorescent tags that can be added to heterogeneously expressed proteins., ProtocolMOREprotein-purification protein-expressionprotocol
Biological BuffersPresented are tables to assist with the selection and preparation of commonly used buffers for use in biotechnology laboratories., ProtocolMOREprotein-expression protein-purificationprotocol
The Naming of Cats (Proteins), MOREblog protein-purification
IMPROvER: The Integral Membrane Protein Stability Selector, MOREblog protein-purification
Measuring Coronavirus Spike:ACE2 Binding Affinity and Kinetics Using the Biacore 8K, MOREblog protein-purification
Coronavirus Replication Machinery: the RNA-dependent RNA PolymeraseIn a previous blog post, we highlighted some excellent recent work that has elucidated the molecular mechanism of host-cell invasion …, MOREblog protein-purification
Dr Steve Harborne SMALP Talk Part I, VideoMOREprotein-purification videosvideo
Does My Protein Look Big in This: Comparisons Between Detergent Solubilised and ‘SMALPed’ GPCRs., MOREblog protein-purification
Dr Steve Harborne’s SMALP Talk Part II – Results, VideoMOREprotein-purification videosvideo
Dr Derek Ogg’s Medicines Discovery Catapult Webinar, VideoMOREprotein-purification x-rayvideo
Do Structures of the Coronavirus Spike Protein Hold the Key to Winning?, BlogMOREprotein-purification x-rayblog
GPCRs: ‘you’ve gotta know how to hold them, you’ve gotta know how to fold them!’, MOREblog protein-purification
Size Exclusion Chromatography: Size Does Matter, MOREblog protein-purification
Thermofluor Stability Assays: More Than Just Thermal Melting, MOREblog protein-purification
Keeping the ‘ions’ in the fireAdvancements in recombinant protein expression technologies allow the production of pretty much any protein or specific protein domains, including modifications …, MOREblog protein-purification
Protein Tags for Expression and Purification – What to use and pitfalls to avoid, MOREblog protein-purification
Protein refolding – easy or excruciating?, MOREblog protein-purification