Protein NMR (Nuclear Magnetic Resonance)

NMR Process Overview

A NMR project will vary depending upon the exact nature of the goal. However a generic workflow is described below.

Construct Design
Construct Design

Construct Design

  • Literature review and Construct Design.
  • Gene synthesis.
Protein Expression
Protein Expression

Small scale feasibility expression & purification

  • Studies in minimal media.
  • Protein QC and analysis.
  • Selection of target construct(s).
Protein Purification
Protein Purification

Scale Up

  • Scale up preparation if required.
  • Expression and purification with 15N, 13C and/or 2H labelled media as required.
Data Processing and Modelling

Data Processing and Modelling

  • NMR data collection at state of the art facility.
  • Data processing and interpretation.

Hear from one of our experts

Mark Elvin: Head of Cell Science

“We provide the full NMR service from construct design through to data processing and analysis. Our scientists access state of the art spectrometers at either Birmingham or Leeds Universities. We have significant experience in producing high quality labelled (15N, 13C and 2H) proteins and can also perform residue specific labelling if required.”

Roberto Maya-Martinez: Senior Protein Scientist

Summary of what we provide

At Peak Proteins we offer NMR to support three aspects of research.

Protein Determination Icon

NMR for Protein Characterisation

Quality control of protein samples.

  • Under different buffering conditions.
  • Or as result of mutations.
  • Changes of compactness as result of binding with other molecules.
  • Determine the presence and quantification of oligomeric species in solution.
  • Performed with unlabelled protein.
Protein Characterisation Icon

NMR to support fragment drug discovery screens

  • Orthogonal high-throughput assessment of fragment compounds.
    • Screening of compound libraries: using cocktails strategy (~10 compounds/cocktail).
    • Hit identification, characterisation, optimisation and validation.
    • Explore low affinity binders: in the range of µM – mM.
  • Generally performed with 15N labelled proteins
Protein Determination Icon

NMR for Protein Structure

  • For 15N or 15N/13C labelled protein.
    • Structure determination of protein alone or in complex with partners.
    • Experience with globular and intrinsically disordered proteins.
    • Backbone assignment H, N, CO, Cα and Cβ.
    • Protein – ligand interactions.

Protein NMR (Nuclear Magnetic Resonance) Resources

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A Comparison of the Structural Techniques used at Sygnature Discovery: X-ray Crystallography, NMR and Cryo-EM, , , , BlogMOREcryo-em blog peak-favourites protein-nmr x-rayblog
Protein and the Planet 2: Directed Evolution of Proteins, , , BlogMOREcryo-em blog protein-nmr x-rayblog
The Magnetic Attraction of Protein NMR – and why you might be drawn to it!, BlogMOREblog protein-nmrblog