With a wealth of experience in the Drug Discovery environment, we are confident our team can fulfil your needs for any part of the Gene to Structure process.
We tailor our professional services to fit your requirements, whatever stage of the process you are at. From construct design, through to expression and purification of your desired protein. Whatever your end use, assay, high throughput screening, biophysics or crystallisation and X-ray structure determination, we can supply your protein in a suitable format.
Peak Proteins’ Services
We use a wide range of affinity and standard chromatography procedures to allow rapid purification of proteins according to their varied and individual characteristics such as size, charge, hydrophobicity.
Robust QC of all reagents supplied is a key part of the service we offer. Size exclusion chromatography, thermal shift and LC-MS are routinely used to characterise and verify the quality of the purified proteins we produce.
Crystallisation is far from predictable, and going from purified protein to crystals suitable for x-ray analysis can often be an arduous journey. With our extensive experience and knowledge of protein reagents, precipitant factors to vary, and the right experimental set-up to use, we aim to generate diffraction quality crystals as efficiently as possible .
Latest News from Peak Proteins
Protein Tags for Expression and Purification – What to use and pitfalls to avoid
In the quest to deliver pure recombinant protein for assay, biophysics or crystallisation, the addition of an affinity tag, to fish out the target protein of interest during purification from [...]
Alderley Park Science Poster Showcase Event 7th Nov
A great opportunity this morning for Juli and Rachel to show case how a combination of protein engineering and detailed mass spectrometry analysis of the expressed proteins can give the [...]
Peak Proteins at Bio Europe 2018
Kicking of November with a couple of European meetings, starting with Mark attending Europe's biggest life science and pharma partnering meeting, Bio Europe 2018 in Copenhagen https://ebdgroup.knect365.com/bioeurope/. Lots of 1-2-1 [...]
Peak Protein Case Studies
Production and analysis of an extensively post-translationally modified secreted protein
We were asked to generate a secreted protein that is known to undergo significant post-translational modification. The challenge was to not only make it but to clearly demonstrate that all the required post-translational modifications had occurred. A HEK293 system was chosen because, as a mammalian cell line, this was most [...]
Production of a post-translationally modified protein
Our client required 20mg of protein for NMR structural studies. The protein was known to be post-translationally modified with palmitic acid on a cysteine residue. Our challenge was to obtain this amount of protein after expression in E. coli and demonstrate palmitoylation using mass spectrometry analysis. The protein [...]
Generation of a novel, secreted protein for structure determination
Our client required >2 mg of a novel, secreted protein for X-ray structure determination studies. The protein was thought to be N-glycosylated. Our challenge was to obtain sufficient >95% pure protein for crystallisation experiments, as measured by SDS-PAGE, size exclusion and mass spectrometry analysis. In addition, we were [...]
