With a wealth of experience in the Drug Discovery environment, we are confident our team can fulfil your needs for any part of the Gene to Structure process.
We tailor our professional services to fit your requirements, whatever stage of the process you are at. From construct design, through to expression and purification of your desired protein. Whatever your end use, assay, high throughput screening, biophysics or crystallisation and X-ray structure determination, we can supply your protein in a suitable format.
Peak Proteins’ Services
Protein Expression and Purification
We design, express and purify customised proteins reagents to support all stages of the drug discovery process. Our cell and protein scientists have a wealth of experience, many from large pharma organisations. We have many peer reviewed publications describing these techniques. We apply all our expertise and knowledge to your protein, providing an interactive, innovative and flexible approach to every project. Every protein and end use is different. We provide a tailored service, taking the time to understand your specific needs to deliver success. We express proteins using a range of platforms including mammalian HEK293 suspension cells, baculovirus/insect cells, and E. coli.
Protein Crystallography and Structure Determination
Our protein structure determination service is driven by an outstanding team of experienced crystallographers with a strong publication record in solving a wide variety of protein structures, including novel ones. A key part of this process is the design of the protein and the ability to screen many different constructs and crystallisation conditions. Our final delivery package is not just a set of co-ordinates. We supply a detailed analysis of the overall 3D structure, along with our learning and conclusions, with ideas for project progression and drug design. Interactive discussions and regular communication with our clients are a key part of our service.
Protein Mass spectrometry
We now provide intact protein and peptide mapping as a stand-alone service to anyone requiring detailed protein or antibody characterisation. Key to this service is the flexibility and quick turnaround times we offer with no detailed contract set-up. Our expert mass spectrometrist, Rachel Rowlinson, will initially discuss your specific needs. Help will be giving with planning and interpretation of the samples analysed and a comprehensive data summary package will be provided with follow-up discussion if required. Whether you have one sample or larger numbers on a regular basis will be reflected in our pricing.
Latest News from Peak Proteins
Thermofluor Stability Assays: More Than Just Thermal Melting
At Peak Proteins, we determine the thermal melting temperature of our proteins as a routine quality control check. Thermal melting can confirm the protein is correctly folded and provide a [...]
Interested in Becoming a COFUND MemTrain Fellow?
The Department of Biosciences at Aston University has recently been awarded the EU Marie Sklodowska-Curie funded COFUND Doctoral Programme entitled MemTrain (Cell Membranes in Industrial Processes Training). Over five years, [...]
Dr Mark Abbott Travels to India to Talk About Protein Mass Spectrometry
Dr Mark Abbott, CEO of Peak Proteins, is travelling to India between the 24th and 25th of June to present a talk on “Mass Spectrometry is an Essential Tool to [...]
Peak Protein Case Studies
Time critical purification of multiple Fab constructs for assay
Time critical purification of multiple Fab constructs for assay As a leading protein purification company, BiVictriX required us to develop a method to purify 10mg each of 24 different antibody-binding fragments (Fabs) to be tested in assays. Once analysed by BiVictriX, a smaller number of chosen constructs were to [...]
Adapting use of size exclusion chromatography to improve yields of an unstable, purified protein
Adapting use of size exclusion chromatography to improve yields of an unstable, purified protein A client asked us to express and purify at least 2 mg each of two similar enzymes for selectivity assays. There was literature precedent for both proteins, and a similar approach would be taken with [...]
Generation of a Nuclear Receptor Protein with known stability issues for SPR studies
Generation of a Nuclear Receptor with known stability issues for SPR studies Our client needed a nuclear receptor protein for Biacore Surface plasmon resonance (SPR) studies. There was precedent for the production of the protein and was available commercially, but it was well known to have stability issues. Our [...]
