Preventing Protein Aggregation to Aid Crystallisation The final purification step prior to protein crystallisation is frequently size-exclusion chromatography in the desired buffer. This enables the purification of a single oligomeric species making successful crystallisation much more likely. A frequent challenge whilst purifying proteins however is their tendency to [...]

Generation of a broad specificity nuclease by refolding of inclusion body expressed protein. Optimisation of downstream processes after cell lysis At Peak Proteins we regularly design DNA constructs for clients, for insertion into mammalian, insect, and bacterial cell systems for over-expression of intracellular proteins. The expressed proteins then [...]

Removing N-linked oligosaccharides to aid protein crystallisation A client was looking to solve the crystal structure of a secreted protein which contained a number of disulphide bonds and potential N-linked glycosylation sites, which leads to the attachment of complex sugar molecules. There was already literature available describing the [...]