With a wealth of experience in the Drug Discovery environment, we are confident our team can fulfil your needs for any part of the Gene to Structure process.
We tailor our professional services to fit your requirements, whatever stage of the process you are at. From construct design, through to expression and purification of your desired protein. Whatever your end use, assay, high throughput screening, biophysics or crystallisation and X-ray structure determination, we can supply your protein in a suitable format.
Peak Proteins’ Services
We design, express and purify customised proteins reagents to support all stages of the drug discovery process. Our cell and protein scientists have a wealth of experience, many from large pharma organisations. We have many peer reviewed publications describing these techniques. We apply all our expertise and knowledge to your protein, providing an interactive, innovative and flexible approach to every project. Every protein and end use is different. We provide a tailored service, taking the time to understand your specific needs to deliver success. We express proteins using a range of platforms including mammalian HEK293 suspension cells, baculovirus/insect cells, and E. coli.
Our protein structure determination service is driven by an outstanding team of experienced crystallographers with a strong publication record in solving a wide variety of protein structures, including novel ones. A key part of this process is the design of the protein and the ability to screen many different constructs and crystallisation conditions. Our final delivery package is not just a set of co-ordinates. We supply a detailed analysis of the overall 3D structure, along with our learning and conclusions, with ideas for project progression and drug design. Interactive discussions and regular communication with our clients are a key part of our service.
We now provide intact protein and peptide mapping as a stand-alone service to anyone requiring detailed protein or antibody characterisation. Key to this service is the flexibility and quick turnaround times we offer with no detailed contract set-up. Our expert mass spectrometrist, Rachel Rowlinson, will initially discuss your specific needs. Help will be giving with planning and interpretation of the samples analysed and a comprehensive data summary package will be provided with follow-up discussion if required. Whether you have one sample or larger numbers on a regular basis will be reflected in our pricing.
Latest News from Peak Proteins
13 is our lucky number!
Whilst 13 might be thought of as unlucky for some, Peak Proteins is delighted to welcome our 13th member to the team! Steve Harbourne joins us following a 3 year [...]
Protein Tags for Expression and Purification – What to use and pitfalls to avoid
In the quest to deliver pure recombinant protein for assay, biophysics or crystallisation, the addition of an affinity tag, to fish out the target protein of interest during purification from [...]
Alderley Park Science Poster Showcase Event 7th Nov
A great opportunity this morning for Juli and Rachel to showcase how a combination of protein engineering and detailed mass spectrometry analysis of the expressed proteins can give the insight [...]
Peak Protein Case Studies
Adapting use of size exclusion chromatography to improve yields of an unstable, purified protein
Adapting use of size exclusion chromatography to improve yields of an unstable, purified protein A client asked us to express and purify at least 2 mg each of two similar enzymes for selectivity assays. There was literature precedent for both proteins, and a similar approach would be taken [...]
Generation of a Nuclear Receptor Protein with known stability issues for SPR studies
Generation of a Nuclear Receptor with known stability issues for SPR studies Our client needed a nuclear receptor protein for Biacore Surface plasmon resonance (SPR) studies. There was precedent for the production of the protein and was available commercially, but it was well known to have stability issues. [...]
Production and analysis of an extensively post-translationally modified secreted protein
Production and analysis of an extensively post-translationally modified secreted protein We were asked to generate a secreted protein that is known to undergo significant post-translational modification. The challenge was to not only make it but to clearly demonstrate that all the required post-translational modifications had occurred. [...]
