Protocols

Lab methods and information that we find useful and thought you might too.

TitleContentCategoriesFile Linkhf:categories
Other Assays To Determine Protein ConcentrationColorimetric and fluorescent assays for protein concentration

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protein-purification
Determining Protein Concentration by Amino Acid AnalysisHow to calculate the concentration of a protein sample using AAA

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Steve’s Pymol Cheat SheetRead Steven Harborne’s recipe for creating great images with PyMol.,

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Western Blotting ProtocolA tried and tested method for Western blots from PAGE.

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Reduction and Alkylation of Protein SamplesA method for the reduction and alkylation of protein samples prior to mass spec analysis

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Protein Tag and Leader Sequence InformationSummary of commonly used tags for protein purification and Secretory leader sequences used for expression in either insect or mammalian …, ,

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Protein Construct Naming ConventionOur in house convention which aims to standardise construct naming and thereby reduce confusion.,

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Determining Protein Concentration by UVAn in depth look at UV absorbance and the equation required to measure protein concentration.,

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Protease InformationProteases are used as tools in protein biochemistry and mass spectrometry experiments. This protocol gives information about their use.,

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protein-purification mass-spec
Mass Spectrometry AidA useful tool summarising masses of amino acids and common modifications found in recombinant proteins.

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mass-spec
Fluorescent Protein TagsSummary information about fluorescent tags that can be added to heterogeneously expressed proteins.,

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DNA FactsheetDNA or RNA concentration and purity estimation using spectroscopy. DNA mobility on Agarose gels.

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protein-expression
Biological BuffersPresented are tables to assist with the selection and preparation of commonly used buffers for use in biotechnology laboratories.,

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