Peak Proteins Protein Science Archives
The global covid-19 pandemic is at the forefront of most people’s minds at the moment, and there has been a monumental effort in the scientific community to try and understand every small detail of the enemy down to the [...]
Walk the line In one of our previous blog posts we discussed the challenges of working with a specific sub-type of membrane proteins called G-protein coupled receptors (GPCRs). All GPCRs have seven transmembrane alpha-helices that span the plasma membrane, [...]
Size exclusion chromatography (SEC), otherwise known as gel filtration chromatography, is one of the simplest and mildest methods of protein separation which is too often thought of as merely a final polishing step in a purification protocol. On the [...]
At Peak Proteins, we determine the thermal melting temperature of our proteins as a routine quality control check. Thermal melting can confirm the protein is correctly folded and provide a measure of how stable it is under a given [...]
Advancements in recombinant protein expression technologies allow the production of pretty much any protein or specific protein domains, including modifications or mutations to the native amino acid sequence. Added to that, the ability to add a range of tag sequences [...]
In the quest to deliver pure recombinant protein for assay, biophysics or crystallisation, the addition of an affinity tag, to fish out the target protein of interest during purification from its crude biological source, is a powerful approach in [...]
In a previous blog, we mentioned the theory of Anfinsen that the structure of a protein is determined solely by the amino acid sequence and that this property enables proteins to be refolded in vitro. If this were universally [...]
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Peak Proteins is a trading name of Sygnature Discovery Limited
