FIDA – Flow Induced Dispersion Analysis

Hear from one of our experts

Mark Elvin: Head of Cell Science

“Fida allows rapid measurement of protein size in solution and can be done with or without labelling. We can use this information to study both functional and structural properties, evaluating protein stability, activity, and ligand binding all using a single instrument.”

Duncan Smith: Lead Protein Scientist

Summary of what we provide

If what you need is not listed below then please contact us, as it is likely that one of our team has experience with the technique you require.

PROTEIN EXPRESSION icon

Flow Induced Dispersion Analysis (Fida)

  • We were the first CRO in Europe to adopt the Fida 1 instrument from FidaBio.
  • Technique for measuring the size of particles in solution.
  • Particle Size: Gives information on:
    • Oligomeric state
    • Aggregation
    • Polydispersity
    • Correlate measured radius to predicted radius from PDB

 

 

PROTEIN EXPRESSION icon

Flow Induced Dispersion Analysis (Fida)

  • Peak Size: Gives information on:
    • Quantification
    • Protein stability
    • Protein absorption
  • Change in signal: Gives information on:
    • Protein-ligand interaction
    • Kd, N
    • Ternary complex analysis
    • Kd1, Kd2, cooperativity
    • Unfolding / denaturation
    • Change in fluorophore environment.

Note we also offer

Surface plasmon resonance (SPR) and Microscale thermophoresis (MST) via the wider group at Sygnature Discovery where we have several BIAcore instruments with a dedicated team of biophysics scientists.

FIDA – Flow Induced Dispersion Analysis Resources

TitleContentCategoriesResources Typeshf:categorieshf:tax:resource_type
PIPS Placement Working on Targeted Protein Degradation, , Blog, Company NewsMOREfida blog protein-purificationblog company-news
Production of Active VHL and Analysis by SPRCase StudyMOREcase-study
Reflections on the Fidabio US User Group Meeting, Company NewsMOREpeak-protein-news fidacompany-news
Applications of FIDA in Protein Science, , PostersMOREfida membrane-proteins protein-purificationposters
Production of Active Cereblon and Analysis by SPRCase StudyMOREcase-study
Revolutionising our Protein Science: How FIDA Enhances Protein Projects at Peak Proteins, , BlogMOREfida blog protein-purificationblog
Steve’s Pymol Cheat SheetRead Steven Harborne’s recipe for creating great images with PyMol., ProtocolMOREfida x-rayprotocol