Mass Spectrometry

Post translational modifications (PTM) play a key part in protein function. One of these is glycosylation, the addition of sugar molecules to a protein by a covalent bond. There are two kinds of glycosylation seen in proteins: N-linked glycosylation and O-linked glycosylation. A further explanation [...]

In this case study Tomas Adomavicius discusses a project where we employed various mass spectrometry techniques to confirm the target API was as expected and also to elucidate the nature of two impurity peaks that were observed during the development of a downstream process [...]

Proximity Based Therapeutics Proximity based therapeutics have emerged as powerful tools in drug discovery and come in a wide range of different formats, including molecular glues and PROTACs (Proteolysis Targeting Chimeras). In all cases a molecule is used to bring together two cellular proteins: [...]

The Name's Bond... Covalent Bond. Covalent compounds have historically accounted for a significant number of successful drugs, but interest waned due to concerns about poor selectivity that is often identified in early assay screening. One of the key hurdles in a covalent drug campaign [...]

Biotinylation of Proteins Biotinylation of proteins is the process of covalently binding a biotin molecule to a protein. At Peak Proteins we can biotinylate purified proteins enzymatically using recombinant BirA or alternatively biotinylate them intracellularly even when secreted. The completion of biotinylation is usually [...]

The Challenge A client asked us to generate Fab and Fc pools from a full-length antibody using Papain digestion. In this case study, Camila Torres, one of our protein scientists describes the issues that we encountered and the conclusion we came to for tackling [...]

Proximity Based Therapeutics Proximity-based therapeutics have emerged as powerful tools in drug discovery and come in a wide range of different types, including molecular glues and PROTACs (Proteolysis Targeting Chimeras). In all cases a molecule is used to bring together two cellular proteins: for [...]

Biotinylation of proteins is a powerful tool for protein science and our recent case study on the in vivo biotinylation of intracellular proteins by co-expression with BirA ligase described how this method is now used routinely at Peak Proteins. A number of our mammalian expressed [...]

A client had requested to obtain a small peptide that was <10kDa, normally processed by cleavage of a larger precursor protein, to be supplied as an untagged recombinant protein. At Peak Proteins, we have experience working with expressing and purifying this type of peptide. [...]

A client had requested us to express and purify a protein for assay studies. Six 6His tagged constructs were designed to increase the chances of obtaining a suitable form of protein that could be tested in assays. After E.coli expression and nickel sepharose affinity [...]