Mass Spectrometry

Proximity Based Therapeutics Proximity based therapeutics have emerged as powerful tools in drug discovery and come in a wide range of different formats, including molecular glues and PROTACs (Proteolysis Targeting Chimeras). In all cases a molecule is used to bring together two cellular proteins: [...]

The Name's Bond... Covalent Bond. Covalent compounds have historically accounted for a significant number of successful drugs, but interest waned due to concerns about poor selectivity that is often identified in early assay screening. One of the key hurdles in a covalent drug campaign [...]

Biotinylation of Proteins Biotinylation of proteins is the process of covalently binding a biotin molecule to a protein. At Peak Proteins we can biotinylate purified proteins enzymatically using recombinant BirA or alternatively biotinylate them intracellularly even when secreted. The completion of biotinylation is usually [...]

The Challenge A client asked us to generate Fab and Fc pools from a full-length antibody using Papain digestion. In this case study, Camila Torres, one of our protein scientists describes the issues that we encountered and the conclusion we came to for tackling [...]

Proximity Based Therapeutics Proximity-based therapeutics have emerged as powerful tools in drug discovery and come in a wide range of different types, including molecular glues and PROTACs (Proteolysis Targeting Chimeras). In all cases a molecule is used to bring together two cellular proteins: for [...]

Biotinylation of proteins is a powerful tool for protein science and our recent case study on the in vivo biotinylation of intracellular proteins by co-expression with BirA ligase described how this method is now used routinely at Peak Proteins. A number of our mammalian expressed [...]

A client had requested to obtain a small peptide that was <10kDa, normally processed by cleavage of a larger precursor protein, to be supplied as an untagged recombinant protein. At Peak Proteins, we have experience working with expressing and purifying this type of peptide. [...]

A client had requested us to express and purify a protein for assay studies. Six 6His tagged constructs were designed to increase the chances of obtaining a suitable form of protein that could be tested in assays. After E.coli expression and nickel sepharose affinity [...]