Protein Expression

In this case study, Emma Cains and Anna Bennett conducted a series of optimisation experiments that led to a ten-fold yield increase of a Biotherapeutic protein. Background Peak Proteins offers CHO, (CHO-3E7) HEK293-6E and HEK Expi293 expression systems for transient mammalian protein production. The [...]

In this case study Sarah Beck outlines our recent success using the BacMam system in HEK293-6E cells to generate high level expression of a GFP-tagged membrane protein required by a client on a large-scale. Introduction At Peak Proteins, as well as producing many classes [...]

HEK293 6E cells (which we have on license from the National Research Council (NRC) in Canada) are a well-established mammalian expression system used at Peak Proteins for expressing recombinant proteins. In this case study Sarah Beck describes the experiments that Calum Wilson our summer [...]

This case study shows how we used mCherry Red Fluorescent Protein to compare recombinant protein expression in Sf9 versus Sf21 insect cells. One of the well-established eukaryotic expression systems used at Peak Proteins for expressing secreted, intracellular and membrane proteins is the flashBACTM baculovirus [...]

Biotinylation of proteins is a powerful tool for protein science and our recent case study on the in vivo biotinylation of intracellular proteins by co-expression with BirA ligase described how this method is now used routinely at Peak Proteins. A number of our mammalian expressed [...]

Our client needed a biotinylated version of a highly disordered protein for their high-throughput screening assay. Previous work with the protein of interest had demonstrated issues with protein stability and loss of protein during concentration using centrifugal concentrators; we were therefore concerned about further [...]

We had been asked by a client to express and purify a protein:Fab complex to support a Cryo-EM project. This was just to be a reproduction of a crystal structure that had been reported in the literature and so on the face of it [...]

A client requested the production of biotinylated and non-biotinylated heterotrimeric complex to support assay screening and biophysics. The protein complex plays a role in tumour suppression, and thus can be used to identify novel targeted therapies. Proteins A and B were cloned into [...]

We were asked to generate a secreted protein that is known to undergo significant post-translational modification. The challenge was to not only make it but to clearly demonstrate that all the required post-translational modifications had occurred. Our Solution An HEK293 system [...]

Our client required 20mg of protein for NMR structural studies. The protein was known to be post-translationally modified with palmitic acid on a cysteine residue. Our Solution Our solutions to this challenge were to reduce the temperature during expression far below [...]