Useful Links

Links to tools and databases we hope you will find useful.

TitleContentCategoriesFile Linkhf:categories
UniProt

UniProt provides the scientific community with a comprehensive resource of protein sequence and functional information.

www.uniprot.org/protein-purification
ExPASy ProtParam Tool

Tool for calculating the properties of your protein sequence (molecular weight, pI, extinction coefficient etc)

web.expasy.org/protparam/protein-purification
ExPASy Translate Tool

Tool to translate DNA sequences in all 6 frames

, web.expasy.org/translate/protein-expression protein-purification
MUSCLE Protein Sequence Alignment Tool

Multiple protein sequence alignment tool

www.ebi.ac.uk/jdispatcher/msa/muscleprotein-purification
RCSB PDB – Protein Structure Database

Online resource powered by the Protein Data Bank providing structural information on the 3D shapes of proteins, nucleic acids, and complex assemblies

www.rcsb.org/x-ray
IMPROvER Tool

Integral Membrane PROtein stability sElectoR. Software that uses three different approaches to identify and rank potentially stabilising mutations in α-helical membrane proteins.

, improver.ddns.net/IMPROvER/membrane-proteins protein-purification
Centrifuge Rotor Speed Calculator Tool

Very comprehensive tool for calculating RCF values for most of the available centrifuge rotors

, www.sciencegateway.org/tools/rotor.htmprotein-expression protein-purification
Protein Solubility Predictor Tool

The protein-sol software will take a single amino acid sequence and return the result of a set of solubility prediction calculations, compared to a solubility database.

, protein-sol.manchester.ac.uk/membrane-proteins protein-purification
MEROPS The Peptidase Database

The MEROPS database is an information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them

www.ebi.ac.uk/merops/protein-purification
IonSource – Mass Spectrometry Resource

Comprehensive educational resource for all things Mass spectrometry

www.ionsource.com/mass-spec
MolView

Molecule drawing and visualisation tool

molview.org/protein-purification