Stephen Moss PhD

Senior Protein Scientist

Stephen has significant experience in construct design, protein expression, purification and characterisation. This is along with experience in protein crystallisation and structure determination and early drug discovery high throughput screening.

Stephen Moss | Peak Proteins

More about Stephen

Stephen graduated from the University of Bath with a degree in Biochemistry.  The degree included an industry placement at Syngenta where Stephen developed a method for characterisation and quantification of fungal lipids using two-dimensional gas chromatography and identified inhibitors of specific fungal protein targets using HTP screening assays.

Stephen’s final year research project was based on expression, purification, and crystallisation of recombinant protein as well as extensive computational modelling and refinement of crystal structures.

Continuing his academic career, Stephen went on to carry out research for a PhD at the University of Bath characterising amyloid-degrading enzymes using molecular cloning, protein expression and optimisation in bacterial, yeast and mammalian cell lines, protein purification using affinity, ion exchange and size exclusion chromatography and protein crystallisation and structural determination using X-ray crystallography.

Stephen joined Domainex after graduating with a PhD, working as a senior protein scientist where he continued to gain experience of design and cloning of target protein gene constructs, the expression, purification, and characterisation of varied protein molecules, crystallisation and structural determination of protein and DNA targets using X-ray crystallography and high throughput screens in the identification of early drug candidates. Stephen was eager to continue working in protein science and structural biology and was delighted at the opportunity to join Peak Proteins

When not in the lab, Stephen enjoys getting outside and can regularly be found hiking or rock climbing in the nearby Peak district or North Wales. More recently, he has been persuaded to join his wife in a local yoga group - he is far from flexible but at least he can now almost cross his legs. If not outside on the weekends, he can be found on the sofa watching Formula 1 and often taking a nap halfway through the race.

High resolution crystal structure of substrate-free human neprilysin
Journal of Structural Biology 2018, Volume 204, Issue 1, pages 19–25 https://doi.org/10.1016/j.jsb.2018.06.004
Stephen Moss, Vasanta Subramanian, K. Ravi Acharya,

Crystal structure of peptide-bound neprilysin reveals key binding interactions
FEBS Letters 2019, 594(2), 327-336 https://doi.org/10.1002/1873-3468.13602
Stephen Moss, Vasanta Subramanian, K. Ravi Acharya,

Employee Spotlight

I am a protein scientist and crystallographer which means I spend my time trying to make very pure protein and crystallise it. If everything goes well, I can use these crystals to determine and analyse the protein’s structure.

I had a similar position purifying and crystallising proteins for another CRO in Cambridge.

Unsurprisingly I quite enjoyed chemistry. I think it was all of the interesting practicals involving a Bunsen burner.

My scientific hero is my science teacher Mrs. Goodear who first sparked my interest in biology and protein structures.

I had heard about Peak Proteins previously. I was interested in joining a company with a welcoming culture and a diverse range of projects. I was particularly impressed by the facilities on offer like the crystal hotels and imagers.

There are many aspects of the job that I enjoy. However, there is something very satisfying about getting distinct peaks on an ion exchange trace. I also really like making figures of protein structures in Pymol and finding the best way to display my results in an accessible and engaging way.

I like to spend my free time outdoors whether it be climbing, walking or biking. I have also begun to manufacture a series of wooden boxes of varying quality to hone my carpentry skills.

Crumbs on the kitchen counter.

Carbonara. There was a period when I found I made this dish far too frequently and had to stop due to a bout of unexpected weight gain.

Some form of exercise. I have been told my temperament is similar to that of a dog in that regard.

My favourite film is the Lord of the Rings: The Fellowship of the Ring and my favourite song is anything off of the Black Keys Magic Potion album.

At one point I could recite the first 53 decimal places of pi.