Protein Science

In this case study, Rachel Johnson describes how we determined the active state structure of the 5-HT2A receptor with the natural ligand serotonin bound using cryo-EM. Introduction GPCRs are a large and diverse group of receptors in the body. They are also important therapeutic targets [...]

Proximity Based Therapeutics Proximity based therapeutics have emerged as powerful tools in drug discovery and come in a wide range of different formats, including molecular glues and PROTACs (Proteolysis Targeting Chimeras). In all cases a molecule is used to bring together two cellular proteins: [...]

In this case study, Toby Allen, one of our protein scientists leads us through the various steps we took to resolve a particularly tricky protein production challenge. We worked on both a full length and truncated version of a DNA binding protein and employed [...]

The Name's Bond... Covalent Bond. Covalent compounds have historically accounted for a significant number of successful drugs, but interest waned due to concerns about poor selectivity that is often identified in early assay screening. One of the key hurdles in a covalent drug campaign [...]

Biotinylation of Proteins Biotinylation of proteins is the process of covalently binding a biotin molecule to a protein. At Peak Proteins we can biotinylate purified proteins enzymatically using recombinant BirA or alternatively biotinylate them intracellularly even when secreted. The completion of biotinylation is usually [...]

The Challenge A client asked us to generate Fab and Fc pools from a full-length antibody using Papain digestion. In this case study, Camila Torres, one of our protein scientists describes the issues that we encountered and the conclusion we came to for tackling [...]

Proximity Based Therapeutics Proximity-based therapeutics have emerged as powerful tools in drug discovery and come in a wide range of different types, including molecular glues and PROTACs (Proteolysis Targeting Chimeras). In all cases a molecule is used to bring together two cellular proteins: for [...]

The effect of ionic strength on protein stability At Peak Proteins we often say that every protein is an individual and they all have their particular nuances and quirks. Once in a while there is a simple solution to a challenge we come across [...]

When your aim is to optimise a particular process, a Design of Experiments (DoE) study is a powerful tool to achieve this in the most efficient manner. In this case study we describe a relatively simple DoE study we performed for a client. However, [...]

A client recently requested the production of two bispecific antibodies and provided us with the heavy and light chain variable sequences that they required. After reviewing the literature, we decided to produce these bispecific antibodies as fused, single-chain variable fragments (scFvs) with an N-terminal [...]