The Name's Bond... Covalent Bond. Covalent compounds have historically accounted for a significant number of successful drugs, but interest waned due to concerns about poor selectivity that is often identified in early assay screening. One of the key hurdles in a covalent drug campaign [...]

AlphaFold AlphaFold is a deep learning system, developed by DeepMind that predicts a protein’s 3D structure from its amino acid sequence. After publication in Nature in 2021, AlphaFold was subsequently used across the entire human proteome. This has since expanded to 48 organisms date which are all [...]

Biotinylation of Proteins Biotinylation of proteins is the process of covalently binding a biotin molecule to a protein. At Peak Proteins we can biotinylate purified proteins enzymatically using recombinant BirA or alternatively biotinylate them intracellularly even when secreted. The completion of biotinylation is usually [...]

HEK293 6E cells (which we have on license from the National Research Council (NRC) in Canada) are a well-established mammalian expression system used at Peak Proteins for expressing recombinant proteins. In this case study Sarah Beck describes the experiments that Calum Wilson our summer [...]

The Challenge A client asked us to generate Fab and Fc pools from a full-length antibody using Papain digestion. In this case study, Camila Torres, one of our protein scientists describes the issues that we encountered and the conclusion we came to for tackling [...]

Proximity Based Therapeutics Proximity-based therapeutics have emerged as powerful tools in drug discovery and come in a wide range of different types, including molecular glues and PROTACs (Proteolysis Targeting Chimeras). In all cases a molecule is used to bring together two cellular proteins: for [...]

The effect of ionic strength on protein stability At Peak Proteins we often say that every protein is an individual and they all have their particular nuances and quirks. Once in a while there is a simple solution to a challenge we come across [...]

This case study shows how we used mCherry Red Fluorescent Protein to compare recombinant protein expression in Sf9 versus Sf21 insect cells. One of the well-established eukaryotic expression systems used at Peak Proteins for expressing secreted, intracellular and membrane proteins is the flashBACTM baculovirus [...]

When your aim is to optimise a particular process, a Design of Experiments (DoE) study is a powerful tool to achieve this in the most efficient manner. In this case study we describe a relatively simple DoE study we performed for a client. However, [...]

A client recently requested the production of two bispecific antibodies and provided us with the heavy and light chain variable sequences that they required. After reviewing the literature, we decided to produce these bispecific antibodies as fused, single-chain variable fragments (scFvs) with an N-terminal [...]