Proximity Based Therapeutics Proximity based therapeutics have emerged as powerful tools in drug discovery and come in a wide range of different types, including molecular glues and PROTACs (Proteolysis Targeting Chimeras). In all cases a molecule is used to bring together two cellular proteins: [...]
Sometimes a dash of salt is all it takes
Richard Mott2023-05-24T15:20:59+00:00The effect of ionic strength on protein stability At Peak Proteins we often say that every protein is an individual and they all have their particular nuances and quirks. Once in a while there is a simple solution to a challenge we come across [...]
Comparing protein expression in Sf9 and Sf21 insect cells
Richard Mott2023-05-24T15:02:07+00:00This case study shows how we used mCherry Red Fluorescent Protein to compare recombinant protein expression in Sf9 versus Sf21 insect cells. One of the well-established eukaryotic expression systems used at Peak Proteins for expressing secreted, intracellular and membrane proteins is the flashBACTM baculovirus [...]
Using Design of Experiments to Improve a Protein Purification Protocol
Richard Mott2023-03-30T12:40:16+00:00When your aim is to optimise a particular process, a Design of Experiments (DoE) study is a powerful tool to achieve this in the most efficient manner. In this case study we describe a relatively simple DoE study we performed for a client. However, [...]
Sherlock Holmes and the Case of the Three Missing Amino Acids
Richard Mott2024-04-22T15:40:33+00:00A client recently requested the production of two bispecific antibodies and provided us with the heavy and light chain variable sequences that they required. After reviewing the literature, we decided to produce these bispecific antibodies as fused, single-chain variable fragments (scFvs) with an N-terminal [...]
In vivo biotinylation of a secreted protein from mammalian cells
Richard Mott2022-11-29T16:28:02+00:00Biotinylation of proteins is a powerful tool for protein science and our recent case study on the in vivo biotinylation of intracellular proteins by co-expression with BirA ligase described how this method is now used routinely at Peak Proteins. A number of our mammalian expressed [...]
In vivo biotinylation of a challenging target in insect cells
Peak Proteins2024-07-05T10:21:45+00:00Our client needed a biotinylated version of a highly disordered protein for their high-throughput screening assay. Previous work with the protein of interest had demonstrated issues with protein stability and loss of protein during concentration using centrifugal concentrators; we were therefore concerned about further [...]
Sherlock Holmes and the Case of the Missing Cysteines.
Peak Proteins2024-04-22T15:41:36+00:00We had been asked by a client to express and purify a protein:Fab complex to support a Cryo-EM project. This was just to be a reproduction of a crystal structure that had been reported in the literature and so on the face of it [...]
Producing Homotrimeric Proteins
Helen Barclay2022-04-26T08:22:53+00:00Many proteins exist and function as multimers in their native state. However, the mechanism by which they achieve this can prove to be an issue when trying to express and purify the protein recombinantly. Two recent projects we have been involved with, required solutions to [...]
Identification of Novel Post Translational Modifications of a Recombinantly Expressed Peptide
Peak Proteins2023-06-19T15:45:54+00:00A client had requested to obtain a small peptide that was <10kDa, normally processed by cleavage of a larger precursor protein, to be supplied as an untagged recombinant protein. At Peak Proteins, we have experience working with expressing and purifying this type of peptide. [...]